eng Ansari Education and Research Society Journal of Ultra Chemistry 0973-3450 2319-8036 January 2018 14 1 26 36 http://dx.doi.org/10.22147/juc/140105 579 article B. SIDDA REDDY 1 SUDHAKAR YADLAPALLI 2 Y. SUBBA RAO 3 P.R. PRASAD 1 K. PRABHAKAR 4 N.Y. SREEDHAR 1 Dept. of Chemistry, S. V. University, Tirupati-517 502, A.P. (India) First Source Laboratory Solutions LLP (Analytical), Hyderabad, T.S. (India) DST-PURSE Centre, S. V. University, Tirupati- 517 502, A.P. (India) Department of Chemistry, DKW Govt. Degree College, Nellore, A.P. (India) <p style="text-align: justify;">A liquid chromatography&ndash;tandem mass spectrometry (LC/ESI/MS/MS) method was developed and validated for the determination of ten sulfonamide antibiotic residues in raw shrimp meat: sulfadimethoxine, sulfachloropyridizine, sulfamethoxypyridizine, sulfamethazine, sulfamethizole, sulfamerazine, sulfathiazole, sulfamethoxazole, sulfadiazine, sulfapyridine. The procedure involves the extraction of homogenized sample into acetonitrile and diluted with mobile phase. The analysis was carried out using ODS-EP 5 micron, 100&times;2.1mm column and mobile phase consists of 0.1% formic acid (A) and acetonitrile (B) in gradient mode at a flow rate of 0.5 mL/min. The final extracts were analyzed by the sensitive and selective LC/ESI/MS/MS operating in positiveion multiple reactions monitoring (MRM) mode.</p> https://journalofchemistry.org/paper/579/ sulfonamides shrimp liquid chromatography-mass spectrometry method development method validation