eng Ansari Education and Research Society Journal of Ultra Chemistry 0973-3450 2319-8036 December 2010 6 3 269 274 juc 584 article Shivaji Bole (shivaji_bole@rediffmail.com) 1 Shachindra. L.N. 1 L.V.G. Nargund 1 K. R. Maruthi 2 Department of Pharmaceutical Chemistry, Nargund College of Pharmacy, Bangalore, Karnataka 560085 (INDIA) Department og Biotechnology, Shri Dharmasthala College of Science, Ujire, Karnataka (INDIA) <p style="text-align: justify;">Alpha amylase was isolated and characterized from <em>Aspergillus niger</em> using source soregum sample. The maximum activity 0.6237&micro;mol/ml/min was obtained and Km value of enzyme was 5.8 x 10^-5. The enzyme of<em> Aspergillus niger</em> could produce alpha amylase under solid state fermentation. The optimal conditions for maximal amylase production, includes pH 6.9, temperature 37^oC and salt 10 Mm concentration. The enzyme activity was determined by DNS method. Using standard maltose curve.</p> https://journalofchemistry.org/paper/584/ enzyme activity