eng Ansari Education and Research Society Journal of Ultra Chemistry 0973-3450 2319-8036 April 2010 6 1 83 88 juc 638 article V. Saleem Basha (saleem_chemistry@yahoo.com 1 K. Hussain Reddy (khussainreddy@yahoo.co.in.com) 2 Government Degree College, Kadiri, Anantapur (INDIA) Department of Chemistry, Sri Krishnadevaraya University, Anantapur-515 003 (INDIA) <p style="text-align: justify;">2-Acetylthiophene isonicotinoylhydrazone (ATINH) has been employed for the direct, non-extractive spectrophotometric determination of mercury. The reagent instantaneously forms a green yellow coloured species (1:2 Hg-ATINH complex) with mercury(II) in acidic medium (pH 6.0, sodium acetate-acetic acid buffer) which remains constant for more than 12hours. The species exhibits maximum absorbance at 420 nm ,with a molar absoprtivity of 4. 1 x 10³L. mol^-1 cm^-1. Beer&#39;s law is valid over the concentration range of 3.2-32.0 ppm of Hg(II). The Sandell&#39;s sensitivity of the complex is 0.875 g cm^-2 of Hg(II). Large number of cations and anions and complexing agents do not interfere in the determination of mercury. The method was successfully applied to biological and soil samples.</p> <p>&nbsp;</p> <p>&nbsp;</p> https://journalofchemistry.org/paper/638/ Spectrophotometric determination mercury 2-acetylthiophene isonicotinoylhydrazone water, biological soil samples