eng Ansari Education and Research Society Journal of Ultra Chemistry 0973-3450 2319-8036 June 2008 4 1 7 14 juc 751 article Anton Smith A 1 Manavalan R. 1 Kannan K. 1 Rajendiran N. 1 2 Department of Pharmacy, Annamalai University, Annamalainagar- 608002 Tamilnadu (INDIA) CAS in Marine Biology, Annamalai University, Parangipettai - 608 502 Tamilnadu (INDIA) <p>&nbsp;</p> <p style="text-align: justify;">&nbsp;</p> <table border="0" cellpadding="5" cellspacing="0" style="width:100%"> <tbody> <tr> <td> <p style="text-align: justify;">A simple and reliable liquid chromatographic method has been developed for the determination of tramadol HCl in the pharmaceutical preparation and biological fluids. The fluorescence of the product was found to have excitation at l_max 272 nm and emission at l_max 298 nm. The method shows high sensitivity with linearity range from 0.07 to 0.4 &micro;g/ml. The lower limit of detection (LOD) was found to be 1.63 x 10^-2 &micro;g/ml, 4.79 x 10^-1 &micro;g/ml and limit of quantification (LOQ) was found to be 4.95 x 10^-2 &micro;g/ml, 1.45x 10^-2 &micro;g/ml in formulation and biological fluids respectively. The different experimental parameters affecting the fluorescence intensity were carefully studied and optimized. The proposed method was applied successfully for determination of tramadol in the pharmaceutical preparation and biological fluids. The percentage recovery was found to be 101.3347 &plusmn;0.7991; 101.196 &plusmn; 1.382 for formulation and biological fluids respectively.</p> </td> </tr> <tr> <td> <p>&nbsp;</p> </td> </tr> </tbody> </table> https://journalofchemistry.org/paper/751/ liquid chromatographic determination of tramadol pharmaceutical dosage