The present study includes isolation, chemical examination nutritional value and medicinal importance of white button mushroom. Significant secondary metabolites were found in Agaricus bisporus after a qualitative phytochemical screening in this investigation. Flavonoids, phenols, saponins, glycosides, and tannins are examples of phytochemical substances that are thought to be important secondary metabolites in mushrooms. Alkaloids, carbohydrates, glycosides, proteins, phenols, flavonoids, saponins, terpenoids, cardiac glycosides, tannins, and steroids were all detected in the extracts during the initial screening process. According to HPLC, the main components in the methanol extract of A. bisporuswere gallic acid and ergothioneine, respectively. These active metabolites are well known for their ability to treat a variety of human conditions, including menstrual disorders, chronic eczema, diarrhoea, dysentery, spasmodic, and diuretic-choleretic. Depending on the molecular formula, retention time-molecular weight, and peak area, our HPLC data indicates the existence of various bioactive substances. Its antibacterial, antidiabetic, and antioxidant qualities may be attributed to the primary phytochemical components found in A. bisporus, which have been identified in the current work.The initial results demand more thorough research, including in vitro tests. Thus, it is possible that the mushroom employed in this study will find application in the pharmaceutical business for a variety of medical purposes.