The aim of the present study is to evaluate various biological activities like antimicrobial, in vitro bovine serum denaturation activity, antioxidant activity for novel substituted fluoro nitro benzothiazolo thioureas. The role of benzothiazole as antitumor agents is well established. The introduction of nitro and fluoro substitution is known to enhance biological activity. Herein, we propose synthesis of some novel compounds for evaluation of various activities.