Crude oil and its different fractions constitute a class of soil pollutants. Under certain conditions, living microorganisms can alter and / or metabolize various classes of compounds present in it, a set of processes collectively called oil biodegradation. This degradation is a process of oxidation which is limited by availability of nutrients and several other factors. In the present experiment, extent of degradation of hydrocarbons of a diesel oil sample was determined in a 90 days experiment gravimetrically by applying it on a soil sample with no past history of oil pollution. Hydrogen Peroxide was applied in one sample. The recovered oils were subjected to GC analysis under identical condition using same column. 25 common peaks have been identified indicating their persistency under the two different conditions.