Piper betel plant is one among the great herbals used in folk and tribal medicines in India. It belongs to the family Piperaceae used as ingredients in a chew commonly known as pan. Chemically piper betel containing chavi betel, chavicol, eugenol, cadinene, alkaloids, sugar, tannin, diastase, betel oil, terpene, sesquiterpene. Biologically Piper betel have broad spectrum activities like antifungal, antibacterial, antitumour, hypotensive, respiratory depressant, anthelmintic, cardiotonic and antifertility. We have used liver slice culture model to demonstrate hepatoprotective activity of PBLE in vitro. CCl₄ (20mM) has been used as a hepatotoxin and the cytotoxicity of CCl₄ is estimated by quantitating the release of LDH in the medium. CCl₄ induces twice the amount release of LDH from the liver as compared to the cells from untreated liver tissue and this was significantly reduced in presence of Plant extract (10µg/ml). Our results clearly point out that PBLE mitigates the CCl₄ induced liver damage by decreasing L