Chemical liquid waste of drug industries contain total coliform (TC) and drug resistance bacteria (DRB). In present study chemical waste is analyzed to determine the total coliform (TC) and drug resistance Bacteria (DRB). Sample were collected from industries outlets, which adjoin in municipal nails and near by and treatment plants situated at mandideep. During sampling, preservation and analysis of samples standard method prescribed by APHA were followed maximum counts of TC (29 X10⁵) reported at after confluence of industrial waste in waste reservoir. while minimum number of TC (15X10⁵) was reported before confluence of drain with water reservoir. DRB increased when chemical effluent mixed with water reservoir.