In the present investigation an attempt has been made to experimentally test the antimicrobial effect of Salvadora persia against four bacterial species, such as Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa and Staphylococcus aureus. The effect of ethanolic extract against the pathogens was tested by disc diffusion technique. The study revealed that, the test organisms were highly susceptible to ethanolic extract of S. persica and this plant can be exploited for developing potential antimicrobial agents.