In (III) has been investigated with Citrulline and Hippuric acid as mixed-ligand complexes at dropping mercury electrode by the polarographic technique. The stability constants of In(III)-Citrulline and In(III)-Hippuric acid were determined first by the DeFord and Hume's method. Later the In(III) was studied with mixed-ligand Citrulline and Hippuric acid by the Schaaf and McMaster's method. The reduction of all mixed-ligand complexes has been found to be quasirreversible and diffusion controlled. Three species of mixed-ligand complexes MXY, MX₂Y and MXY₂ have been determined and found to be more stable than that of simple complexes. The detailed temperature effect has been also studied.

Bis-azo acid dyes were prepared by the coupling of 4,4'-methylene bis(anthranilic acid) with various pyrazolone coupling components. The resultant dyes were characterized by elemental analyses, IR and NMR spectral studies. The UV-visible spectral data have also been discussed in terms of structural property relationship. The dyeing assessment of all the dyes was evaluated on wool and nylon textile fibres. The results show that a better hue was obtained on wool and nylon fibers. The results of the anti-bacterial properties of the bis-azo acid dyes revealed that the toxicity of these dyes against bacteria is fairly good.

Alpha amylase was isolated and characterized from Aspergillus niger using source soregum sample. The maximum activity 0.6237µmol/ml/min was obtained and Km value of enzyme was 5.8 x 10^-5. The enzyme of Aspergillus niger could produce alpha amylase under solid state fermentation. The optimal conditions for maximal amylase production, includes pH 6.9, temperature 37^oC and salt 10 Mm concentration. The enzyme activity was determined by DNS method. Using standard maltose curve.

Modified electrophoresis technique viz. solution electrophoresis has been employed for the study of the metal ligand equilibira in solution. The method is based upon the migration of metal ion under electric influence in electrophoretic tube with variation in pH of the black ground electrolyte (ionic strength 0.1). The plot of pH Vs absorbance difference reveals the nature of complexation and help in calculating its equilibrium constants. The present work report the result on the binary viz. Cu(II), Ni(II), Co(II) and Zn(II)-Glycyl Glycine complexing system.